![]() ![]() Data are available in Supplementary Table 2. d, Distribution of cell populations, identified from nuclei within atria (left and right) and ventricles (left, right, apex and interventricular septum) after subclustering analysis. c, Uniform manifold approximation and projection (UMAP) embedding of 487,106 cells and nuclei delineate 11 cardiac cell types and marker genes. ![]() Data are available in Supplementary Table 1. b, Infographic shows donors (women, top men, bottom), age, and contribution to cells and nuclei datasets (orange circle). Single nuclei ( n = 14) and single cells ( n = 7) were processed using Chromium 10x 3′DEG chemistry. Our human cardiac cell atlas improves our understanding of the human heart and provides a valuable reference for future studies.Ī, Transmural samples were obtained from left and right atrium, left and right ventricles, apex and interventricular septum from 14 individuals. Furthermore, analyses of cell-to-cell interactions highlight different networks of macrophages, fibroblasts and cardiomyocytes between atria and ventricles that are distinct from those of skeletal muscle. In the immune compartment, we identify cardiac-resident macrophages with inflammatory and protective transcriptional signatures. We define the complexity of the cardiac vasculature and its changes along the arterio-venous axis. Our results highlight the cellular heterogeneity of cardiomyocytes, pericytes and fibroblasts, and reveal distinct atrial and ventricular subsets of cells with diverse developmental origins and specialized properties. Here, using state-of-the-art analyses of large-scale single-cell and single-nucleus transcriptomes, we characterize six anatomical adult heart regions. Knowledge of the full repertoire of cardiac cells and their gene expression profiles is a fundamental first step in this endeavour. Advanced insights into disease mechanisms and therapeutic strategies require a deeper understanding of the molecular processes involved in the healthy heart. Nature volume 588, pages 466–472 ( 2020) Cite this articleĬardiovascular disease is the leading cause of death worldwide. “We thank the NeuroTechnology Studio at Brigham and Women’s Hospital for providing instrument access and consultation on data acquisition and data analysis. For more information please contact Lai Ding ( ACKNOWLEDGEMENT: If support from the NeuroTechnology Studio results in a research paper or other public presentation, please acknowledge this support by including the following statement in your publication(s): Each lab has a “super user” who was trained by 10X Genomics, who will then train lab members. For training on this instrument, please contact your lab’s PI. Booking and pricing information are available through the Partners Core Management System.Īs an alternative for users withing to run their own experiments, the Neurotechnology Studio also has a stand-alone 10X controller, located in the Studio’s own lab (HBTM 07006D). ![]() Please contact the core for further details. ![]() This is now a separate core facility that provides a full pipeline from cell sorting to library preparation to sequencing and bioinformatic analysis. First, the studio partnered with the Division of Rheumatology Alllergy and Immunology and the Evergrande Center to launch a full-service Single Cell Genomics Core. Users interested in the 10X Chromium technology have two alternatives. The Chromium Controller fits on a standard laboratory bench and allows a user to run any Chromium Solution, from genome to single cell analysis. The compact, sleek Chromium Controller rapidly and efficiently combines large partition numbers with a massively diverse barcode library to generate >100,000 barcode-containing partitions in a matter of minutes. ![]()
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